Yes, in most mario games you have to "kill" enemies, but in mario kart you don't, although we might question whether in mario games you are actually killing something.
Mario definitely kills the turtles and the other enemies in the normal platform games.
In Mario Kart I don't think they die because they always come back with the cloud, so they are never killed. This is probably because the game is a racing game, where there aren't any kills usually in that genre.
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